Bead separation. GFP-Trap® Dynabeads（磁気ビーズ標識GFP抗体） 商品名: GFP-Trap® Dynabeads（GFPトラップ®ダイナビーズ） （Nano-Trap® alpaca anti-GFP, recombinant VHH, coupled to Dynabeads） 2007). The binding rate of GFP-Trap however is very high. Anti-DYKDDDDK Kit for Immunoprecipitation allows a rapid and efficient immunoprecipitation and elution of an active DYKDDDDK -tagged recombinant protein in less than 2 hours. 4th … Casp2pro BiFC cells were again treated with caspase‐2‐activating stressors, lysed, and incubated with GFP‐Trap. All in less than 4 hours. 纯度不高. For biochemical analysis including mass spectrometry and enzyme activity measurements, TurboGFP-fusion proteins and their interacting factors can be isolated fast and efficiently by immunoprecipitation using the TurboGFP-Trap. The ability to specifically capture caspase‐2 dimers afforded an optimal system to isolate and characterize the interactome of dimerized caspase‐2. Le GFP-Trap® est une matrice monovalente (agarose ou billes magnétiques) couplée à des protéines qui se lient aux GFP. Comparison of GFP-Traps® with conventional mono- and polyclonal antibodies. 同 B. GFP-Trap®_A manual - 1 - Version 2017-10-04 GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. Only for research applications, not for diagnostic or therapeutic use 1. gta. gfp 2,489 citations have been found for this product All applications Affinity chromatography (Affin Chroma) Binding experiments (Binding) Chromatin immunoprecipitation (ChIP) Functional assays (Functional) Immunocytochemistry-immunoflourescence (ICC-IF) Immunopurification (Immu-puri) Immunoprecipitation (IP) Pull-Down Assay (Pull-Down Assay) RNA Immunoprecipitation (RNA-IP) … Add to Basket Add to Quote INQUIRE FOR PRICING. As expected, the bound fractions containing GFP are extraordinarily pure. GFP-Trap®_A manual - 1 - Version 2017-10-04 GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. Introduction Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. 10 LIST OF ABBREVIATIONS Adh ARC CCA1 ChIP c-Myc CRSP CTD DRIP EST GFP GUS HAT Hsp18.2 IgG PC2 PIC RNA pol Sep10 SMCC Soh1 SWI/SNF TAP TFIIA TFIIB alcohol dehydrogenase activator-recruited cofactor circadian clock associated 1 chromatin immunoprecipitation cellular myelocytomatosis oncogene cofactor required for Sp1 activation carboxy-terminal domain vitamin D receptor interacting … Affinity purification ChIP and related techniques ELISA GFP High content analysis Immunofluorescence Immunoprecipitation Ligand capture and Affinity Determination Methods Live Cell Imaging Mass spectrometry On-bead enzyme assays Spot Capture and Detection System Super … The GFP-Trap® is a monovalent matrix (agarose or magnetic beads) with GFP-binding proteins coupled to it. We describe the GFP-Trap immunoprecipitation as a cell-based method to analyze the OTUD6B-OTUB1 interaction in the cellular context and the AlphaScreen (amplified luminescent proximity homogeneous assay) assay as a tool to detect direct interactions and to search for PPI inhibitors. For a specific protein or complex, the optimal experimental conditions/ GFP-Trap have to be tested. Enlarge Image × Immunoprecipitation of GFP with GFP-Trap Agarose beads. What GFP-Trap should I use for my immunoprecipitation? No binding to proteins derived from DsRed, all RFPs and TurboGFP can be detected. Proteintech North America (HQ) Proteintech Group, Inc 5400 Pearl Street, Suite 300 Rosemont, IL 60018, USA. Size GFP-tagged protein*. GFP 抗体-琼脂糖珠子， RFP-Trap ， GST-Trap ， Dnmt1-Trap. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. GFP-Trap® specifically binds to eGFP, wtGFP, GFP S65T, TagGFP, eYFP, YFP, Venus, Citrin, CFP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (magnetic agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. Low background*. Only for research applications, not for diagnostic or therapeutic use 1. Dynabeads M-270 Bead form. The combination of low disscociation and high binding rates results to a very low K D respectively high affinity. iST-GFP-Trap Kit for immunoprecipitation and sample preparation for mass spectrometry. scrambled miR or miR-29a (Karginov et al. Immunoprecipitations (IP) of GFP from protein extracts of human cells. Immunoprecipitation is a highly selective technique which can be used to enrich or concentrate low abundance species from a complex matrix. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. The immunoprecipitation is performed with anti-DYKDDDDK® antibody coupled to agarose beads, which are generated by covalently linking agarose to a highly specific mouse monoclonal antibody raised against DYKDDDDK. GFP-Trap for Immunoprecipitation Anti-GFP antibody to perform immunoprecipitation of GFP fusion proteins and their interaction factors. • More than 1,500 publications. • Reduced background signal. Ligand. The GFP-Trap has a very low dissociation rate; only very small amounts of GFP dissociates even during lengthy washing. GFP-Traps® are ideal for ... RNA Immunoprecipitation (RIP) GFP-Trap® - Single band purification of GFP. GFP-Trap is an anti-GFP antibody fragment conjugated to magnetic beads. Applications. 产物. 通用方法，适合面宽. 免疫沈降（IP; ImmunoPrecipitation）は抗原と抗体の親和性を利用して、溶液中から抗原を特異的に分離させる方法です。免疫沈降で分離した抗原はSDS-PAGEやウエスタンブロッティングなどの方法で解析します。 原理. 1-888-478-4522 proteintech@ptglab.com . GFP‐Trap immunoprecipitation of caspase‐2 BiFC dimers identifies active caspase‐2‐interacting proteins. More than 1,800 publications peer reviewed articles have already been published using ChromoTek's GFP-Trap. Introduction Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. GFP-Trap®_MA kit manual - 1 - Version 2017-10-04 GFP-Trap®_MA for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. Immunoprecipitation is a common first step for experiments such as selectively enriching for phosphoproteins or peptides for post-translation modification studies or concentrating a biomarker prior to quantitation. Proteintech Europe. I am encountering a weird problem in my immunoprecipitation experiments with GFP magnetic beads. Introduction localization and dynamics. Freight/Packing:-Country/Region. Quantity. GFP-Trap’s affinity has been measured as 1 pM (Figure 4). 适用性. The complex is removed from the solution manually against … Introduction localization and dynamics. Turbo-GFP shares only ~21 % sequence identity with the commonly used GFP variants. GFP-Trap For immunoprecipitation of GFP-fusion proteins Referenced in about 1,600 publications. Produktmerkmale Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. 同 B. GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. I: Input, FT: Flow-Through, B: Bound. Les GFP-Traps® sont idéales pour l'isolation efficace en une seule étape des protéines de fusion fluorescentes et des partenaires qui interagissent avec elles (voir le schéma global ci-dessous). GFP-Trap®_A manual - 1 - Version 2009-09-28 GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins (gta) For the immunoprecipitation of GFP-fusion proteins from cellular extracts. Its advantages are as follows: • Extremely efficient reduction of GFP fusion proteins. Immunoprecipitation of GFP with GFP-Trap Agarose beads. GFP-Trap®_MA manual - 1 - Version 2017-10-04 GFP-Trap®_MA for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (magnetic agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. Immunoprecipitation For the c-myc-Ago2 immunoprecipitation ex periments, rat Schwann cells wer e transfected with the c-myc-Ago2 plasmid and either the negative (Neg.) .. Co-transfection of vMIA-myc and GFP-N1 was used as negative control. GFP-Trap. Based on the properties of the different matrices (see table), we recommend: GFP-Trap Agarose for very low background and high binding capacity IP; GFP-Trap Magnetic Agarose for magnetic separation and high binding capacity IP; GFP-Trap Dynabeads for IP of very large proteins/complexes; GFP-Trap Multiwell … 同A. The PreOmics / ChromoTek iST-Trap Kit enables the fast and efficient immunoprecipitation of GFP-Fusion proteins and the interaction factors followed by the innovative sample preparation for mass spectrometry based proteomics. For IP, the Immunomagnetic Beads are added to a sample containing GFP-tagged proteins to form an Immunomagnetic Beads-protein complex. Load more search results . Immunoprecipitation with GFP-Trap. GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins For the immunoprecipitation of GFP-fusion-proteins from cellular extracts. For immunoprecipitation of Pex19-YFP the GFP-Trap_M kit (Chromotek, Planegg-Martinsried, Germany), consisting of a high quality GFP-binding protein coupled to a monovalent matrix of magnetic agarose beads, was used. 同A. 可选用特异性抗体 或 GFP 单克隆或多克隆抗体 +Protein A/G 琼脂糖微珠. The ChromoTek GFP-Trap provides fast, reliable, and e ffective immunoprecipitation of GFP-fusion proteins. SKU/Size. 有 GFP ， RFP ， GST ， Dnmt1-Trap 等 4 个品种的标签蛋白 Trap 可选. Introduction localization and dynamics. In... Matrix. Binding capacity. Magnetic GFP-Trap-M Kit, for GFP Fusion Protein Immunoprecipitation (20 tests) CHT gtmk-20 B 1 kit / 77,000 GFP結合ビーズ別売品 品 名 メーカー 商品コード 包装 /価格（￥） GFP-Trap-A CHT gta-20 20 tests / 61,000 Magnetic GFP-Trap-M CHT gtm-20 20 tests / 61,000 抽出 精製 使 用 例 I: Input, FT: Flow-Through, B: Bound. The GFP Tag Immunomagnetic Beads, conjugated with GFP Antibody（13105-R208）, are used for Immuneprecipitation / IP of GFP-tagged proteins expressed in vitro expression systems. I: Input, FT: Flow-Through, B: Bound.-/ -Cat No. Choose from 3 GFP-Trap (50 µL, 2 reactions) sample types: • GFP-Trap Agarose (for very low background and high binding capacity IP) Introduction localization and dynamics. The same 1-step purification procedure was then applied to GFP fused to the protein PCNA in a co-immunoprecipitation experiment.
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